FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25000734
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG30400.022.41450328
ACGTCTG31550.021.73813615
CGTCTGA34200.019.92224916
ACGTCCG35950.019.17193632
TCGTATG33350.018.78046845
CTCGTAT35100.018.10101344
GTCCGCA37950.017.98418834
GTCACGT44850.015.50424929
CCGCACA44400.015.16905836
TCGGAAG47500.014.8171533
ATCGGAA48100.014.7258792
TCACGTC49300.014.01738530
CACACGT54600.013.17729212
CACGTCT53850.013.11072514
CAGTCAC56850.012.85428227
TCCGCAC56800.012.61068735
CGCACAT57050.011.92430337
TCTCGTA60950.011.01548143
ACACGTC67950.010.88632713
ATCGGTG79300.010.3771596
Produced by FastQC (version 0.11.5)