FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25793366
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40300.024.44006315
CGTCTGA43200.022.59128416
CTCGTAT35700.021.07715844
GTCCGCA45650.020.67359436
TCGTATG36400.019.24816945
ATCGGAA54200.018.2968312
TCGGAAG56550.017.6557483
CACGTCT56700.017.64736614
CAGTCAC62000.016.03040327
CGCATCT56300.016.00562939
CACACGT62750.015.98124412
ACCCGTC62750.015.39764332
TCCGCAT62700.015.08804437
TCACCCG68300.014.23644130
ACACGTC73950.014.13874813
AGTCACC74100.013.90014328
CCGTCCG78900.013.10292634
CCCGTCC78750.012.75569933
GTCACCC79900.012.30739329
CGTCCGC86350.012.2593735
Produced by FastQC (version 0.11.5)