FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23717402
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49850.029.18551315
CGTCTGA53200.027.55904416
ATCGGAA62550.023.6552562
TCGGAAG63350.023.391933
CACGTCT67450.022.23505214
CACACGT71700.020.60286912
CAGTCAC72000.020.39335627
GTCAGAA70700.020.23079336
CTCGTAT34950.020.1761544
TGTCAGA72500.020.00783235
GTCACAT73300.019.86252429
TCGTATG32000.019.57182745
AGTCACA76850.018.87444728
ACACGTC82150.018.6938713
CCAGTCA86250.016.89302626
AATCTCG60300.016.81265641
ACATGTC89300.016.57113632
GATCGGA90850.016.535011
TCACATG88800.016.47610530
AGCACAC92800.016.37869510
Produced by FastQC (version 0.11.5)