FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29532913
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG44500.028.39530434
TCCGTAT44450.027.56702637
CGTATCT43650.027.5086439
ACGTCTG48900.026.21151415
CCGTATC47500.026.08358238
CGTCTGA51100.025.08303316
TTCCGTA53550.023.17648536
CTCGTAT46500.022.62615444
TCGTATG44550.022.50369845
GTTCCGT63800.019.84083735
ATCGGAA67650.019.1464422
TCGGAAG69650.018.7579883
CACGTCT71100.018.53194614
TATCTCG63250.017.91654441
CACACGT75100.017.54444712
GTCACAG76350.017.0652529
TCACAGT74650.017.00585430
CAGTCAC78750.016.41806827
CACAGTT80800.016.24706531
AGTCACA81950.015.97112128
Produced by FastQC (version 0.11.5)