FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22079996
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG36750.026.55698215
TCGTATG33100.025.11363645
CGTCTGA39650.024.38785216
CTCGTAT36850.023.10832644
ATCGGAA49050.019.9889892
TCGGAAG49300.019.9331933
CACAGTC49550.019.4941931
CACGTCT53550.018.60176714
CACACGT56300.017.9321412
GTCACAG56300.017.42687829
TCACAGT56600.017.33863430
CAGTCAC57700.017.26420827
AATCTCG55150.017.03353341
AGTCACA58450.016.73665828
AGTCAAC64000.015.62512834
ACACGTC67050.015.45974213
ACAGTCA67350.015.0481732
GTCAACA69750.014.14332335
TCTCGTA63400.014.07084343
GATCGGA73650.013.6181241
Produced by FastQC (version 0.11.5)