FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15050766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN364930.24246606451791222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG4200.032.09928529
GAGTGTA4300.031.8749128
TCTCGGT4400.031.2621237
GTAGATC4550.030.62196232
GTGTAGA4700.028.68695330
CACTCTT8550.028.4076252
TAGATCT4900.027.97605733
TGTAGAT5500.024.51553231
GATCTCG6350.022.29584535
GTGTAGG6600.021.45159117
CTCGGTG6450.021.37086538
TCGGTGG7050.020.1969839
TTCCCTA4900.020.1701038
GTAGGGA6800.020.16728819
TAGGGAA6900.019.87494320
AGATCTC7150.019.80100634
TCGTGTA7150.019.79691315
TGTAGGG7400.018.83584618
TCCCTAC4300.017.7603039
AAGAGTG8400.016.85437226
Produced by FastQC (version 0.11.5)