FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24155003
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN306510.12689296705945347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25800.016.0580522
GCGTGAC35650.012.2526161
GTGACTC36000.011.7573773
GTAGAAC32000.011.10156811
GTACTCG52950.010.8417314
TACTCGA55300.010.2572575
AGTACTC62150.09.4910723
CTAGCAC46100.09.46430814
GCTCTAG45250.09.34149611
CTCGAAT22300.09.2890141
TGACTCT51600.08.94393354
AATCGAA44850.08.8329565
GGAAAAT98600.08.7916521
GAATCGA40600.08.7055864
GAATTGA61050.08.5920084
AAAATCC73850.08.5056743
TAGAACA41800.08.4452812
TCAGGTA44400.08.4074717
GGTAGAA43350.08.2468310
CACGGTA16250.07.75405919
Produced by FastQC (version 0.11.5)