FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26422915
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45350.011.353147511
TCAGGTA61050.09.3187047
GGTAGAA57450.09.31425910
CAGGTAG58750.09.2237698
TAATCGA30050.09.2142174
GAATCGA47500.09.0044634
TAGAACA58350.08.823873512
AAAATCC91850.08.7258773
GGAAAAT127500.08.7235941
CTAATCG27400.08.3808463
AAATCCT94950.08.1082854
AATCGAG50700.08.0354765
GGGGAAT54050.07.8314251
GGGAAAT60500.07.6663831
AATCGAA56050.07.5897445
AGGTAGA71700.07.494749
CATAAGC88300.07.486997
CTCGAAT29800.07.40437651
CGAATCG32300.07.31854963
AGATCAG109700.07.3053534
Produced by FastQC (version 0.11.5)