FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27527288
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT116300.08.6925141
CGTAACG27300.08.57602742
GGTAAGT30600.08.30778517
GGGGAAT70150.07.86352351
GAATCGA46500.07.5523264
GTAGAAC36800.07.515045611
AAAATCC88350.07.49195243
AAAAATC101300.07.44601062
AAATCCA103900.07.4316914
GCGTAAC31050.07.39489241
GCGAAAT62000.07.2630171
GAATTGA80050.07.1148914
AATCGAA58850.07.07575275
TTCCGAC33600.06.964531423
CGAAATT49750.06.83395532
TACGCAC14950.06.7680189
GAAAAAT157500.06.73316341
GGAGAAT101700.06.7081141
ACATGCG36650.06.69028046
AATCGAG54050.06.66302825
Produced by FastQC (version 0.11.5)