FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25928791
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37650.010.15229511
CGTGACT20950.010.1025792
CTCGAAT27050.09.4886261
GTACTCG49250.09.1876654
GAATCGA47950.08.9672664
AATCGAA49400.08.7028115
TACTCGA51350.08.6791635
GAATTGA69800.08.4500874
GGAAAAT109300.08.2601881
GGTAGAA48400.08.22264610
AGTACTC61700.08.1735543
GGGAAAT57450.08.1515271
TATAAGG28500.07.9768175
AATTGAC45550.07.95592365
TCAGGTA51500.07.9470317
AAAATCC76050.07.8746363
TAGAACA48300.07.820840412
AAAAATC77500.07.75707442
GGTAGGC25800.07.58298737
GCGTGAC30500.07.52949861
Produced by FastQC (version 0.11.5)