FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27712109
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24900.012.7500062
GTACTCG63700.011.5562774
GTAGAAC40750.011.53180111
TACTCGA65300.011.0300855
AGTACTC74450.010.4631563
GAATCGA49150.09.89327054
GCGTGAC34600.09.7610111
GGAAAAT113450.09.4864351
CTCGAAT26900.09.0396391
GTGACTC36300.08.9311473
TAGAACA53750.08.91015212
TCAGGTA56300.08.7871447
GGTAGAA53750.08.78457810
AAAATCC91000.08.6344633
GAATTGA70100.08.4780424
TATAAGG31150.08.3818895
AATCGAA59050.08.1571035
ACGGTAC18650.08.08325620
CTATAAG30400.08.0716544
AAATCCT95050.07.91050054
Produced by FastQC (version 0.11.5)