FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27168898
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27800.012.3106732
GTAGAAC31300.09.98504411
GTGACTC38450.09.3098723
GTACTCG56550.09.2752444
GCGTGAC39950.09.2426481
TACTCGA55300.09.1985885
GGAAAAT114450.08.7344691
GGGAAAT56400.08.4630351
GAATCGA47900.08.459394
AGTACTC65500.08.3867063
AGCCTAG30300.08.24226725
AAAATCC87200.08.1069233
CTCTATA55300.07.982458645
CTAGCAC44150.07.946764514
AATTGAC43950.07.88678035
CACTCGT56100.07.699437635
AATTCAC40850.07.65879775
GAATTGA66250.07.6113934
TATAAGG30250.07.51508435
GCGGAAA68650.07.51042181
Produced by FastQC (version 0.11.5)