FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002194
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT110300.09.28799251
GAATCGA38300.08.9928984
GTAGAAC33100.08.89855711
CGCGATA14700.08.87544329
AAAATCC83150.08.5289963
CGATAGT15600.08.36399331
AAATCCT81600.08.0556334
AATCGGT18900.07.86008745
TACCCTA22400.07.6289139
AATTCAC42700.07.59066635
CGTAACG22400.07.537471342
GGACTTA30000.07.42883874
TATAGAA38800.07.1957481
GGGAAAT64600.07.17993741
GAAAATC93900.07.1456562
AATCGAA51400.07.1378895
GCGAAAT48600.07.1346131
GGTAGAA44850.07.11875310
GGTAAGT23800.07.08973417
GGAAGAT164550.07.07422161
Produced by FastQC (version 0.11.5)