FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26785156
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26500.014.0196292
GCGTGAC37300.010.3824781
GTGACTC37800.010.3637483
GGAAAAT121250.09.897531
GTACTCG61250.09.40916254
AAAATCC91050.09.3469783
TACTCGA61300.09.2898345
AGTACTC70300.08.9032553
GTAGAAC33150.08.88518911
AAATCCT91850.08.6519234
GAATTGA67950.08.6470534
AATTGAC46850.08.4076845
CTATAAG27800.08.0978984
AATCGAA51700.08.0978615
GGGAAAT61250.08.0871951
GCTCTAG48000.08.05686611
CTCGAAT26350.07.9466431
CTAGCAC46150.07.943617314
GACGTAA9350.07.940796425
GGCGAAT18600.07.8683151
Produced by FastQC (version 0.11.5)