FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n02_A3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n02_A3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24700518
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25650.012.201782
TACTCGA55350.010.6949695
GCGTGAC33100.010.6116441
GTACTCG56450.010.6077554
AGTACTC64600.010.0023063
AAAATCC83250.09.5464353
GGAAAAT112500.09.3865591
GTAGAAC31300.09.338711
GAATCGA39700.09.1860824
CTCGAAT26400.09.0404071
GTGACTC36400.08.9065993
AATTGAC44150.08.7177975
GAATTGA67650.08.618624
AAATCCT84250.08.256474
CACTCGA31750.08.0828944
GAAAATC92000.08.0764752
AGTGTGA54350.07.86236814
AATCGAA46500.07.8415785
ATTGACC42750.07.8405336
TCACACG25150.07.78082315
Produced by FastQC (version 0.11.5)