FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15050766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG3600.033.7152315
CGTCTGA3800.032.53245516
CACGTCT4450.028.28440314
ACACGTC4650.026.10081313
CACACGT4650.026.10081312
ACCGATC5450.025.20284534
AGACCGA5750.023.88544732
CCGAGAC5850.023.48324629
GAGACCG5850.023.47699431
AGCACAC6100.020.26369710
CGAGACC7250.019.5804430
TACTATT1901.8189894E-1218.92665518
CAGTCAC4550.018.79658327
CCAGTCA4700.018.67474226
GGAATGC7650.018.23104523
GCACACG7200.018.10506411
AGCGGTT7900.017.63880511
CCGATCT7850.017.49833135
AGGAATG8100.017.48830422
TATAGGC2057.4578566E-1116.4681227
Produced by FastQC (version 0.11.5)