FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24155003
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC39750.025.44924738
ACGTCTG43000.024.94255315
CCGTACG43200.024.35206633
CGTCTGA44300.023.90636316
CTCGTAT37750.023.69714544
TCGTATG37700.022.59290345
ACCGTAC48700.021.97006432
CACCGTA52550.020.18385531
TACGTAA53300.019.23205236
CACGTCT57100.019.0585714
ATCGGAA57100.018.8623872
GTAATCT54150.018.72423639
TCGGAAG57900.018.6405623
ACGTAAT55300.018.29217537
CACACGT60350.017.84571512
AATCTCG63250.015.81739741
CAGTCAC74550.015.62214627
ACACGTC71250.015.36808613
TAATCTC67550.015.14405540
AGTCACC81350.014.53917828
Produced by FastQC (version 0.11.5)