FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26422915
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG43700.032.9875333
ACGTCTG52500.028.4397315
AGTCACG51350.028.3782628
ACGTTTC53750.027.02741632
GTTTCGG53700.026.76172834
TTCGGAA54050.026.33963236
CGTCTGA57400.026.05136716
TTTCGGA56750.025.08637235
GTCACGT60600.024.02121729
CACACGT63400.023.6557112
ATCGGAA66050.022.469892
TCGGAAG68200.022.190083
CTCGTAT43400.021.85553244
TCGGAAT66400.021.67899937
TCGTATG43850.020.55217445
CACGTTT71500.020.47011631
CACGTCT76050.020.34195714
ACACGTC80000.019.22496413
CGGAATC76350.018.64776838
GGAATCT84100.017.11794539
Produced by FastQC (version 0.11.5)