FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27527288
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58450.027.85166415
AGTCACG58350.027.3621928
CACGTGG61150.026.21967131
GTCACGT61850.025.86350629
CGTCTGA63300.025.7890616
TCGGAAG71900.022.9862523
CTCGTAT57250.022.7094444
ATCGGAA72850.022.6247652
ACGTGGC72650.022.41541332
TCACGTG74350.021.74002330
CACACGT76700.021.48785412
TATCTCG70250.020.75166741
TCGTATG60800.020.56812545
CACGTCT80250.020.5374514
GGCCTTA78050.020.43500536
ACACGTC83700.019.9862813
GCCTTAT80200.019.63495337
GATCGGA93300.017.6911011
CGGAAGA98200.017.265124
CTTATCT92900.016.90386439
Produced by FastQC (version 0.11.5)