FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25928791
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG41850.022.1370428
TCGTATG41750.021.47998445
ACGTCTG47300.020.34590315
CGTGAAA46050.020.3009733
CTCGTAT42600.020.15225444
ACGTGAA47700.020.11643232
CGTCTGA50300.019.17739116
CACGTGA50100.018.8787131
AACGATC47350.018.08071538
GTCACGT54700.017.3983829
TGAAACG54650.016.90141335
CACGTCT63400.015.63970714
AAACGAT58750.015.530601537
CACACGT63800.015.36520512
TCGGAAG68150.014.6163483
ATCGGAA67800.014.5923382
TCACGTG69450.014.06796930
GAAACGA66600.013.80117736
ACACGTC77350.013.05144513
CAGTCAC80050.012.75144927
Produced by FastQC (version 0.11.5)