FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27712109
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG33700.018.41557715
AGTCACG34550.016.85722528
CGTCTGA37000.016.59109116
CTCGTAT34100.015.9901644
ACGTCCG37150.015.94572532
TCGTATG35950.014.791224545
GTCCGCA42000.013.94492334
GTCACGT44600.013.36860329
CCGCACA45700.013.21076536
CACGTCT52000.012.280333514
TCGGAAG52700.011.9467473
ATCGGAA52000.011.8048782
CACACGT54550.011.66484512
TCACGTC55700.010.87232230
TATAGGC51000.010.7571427
TCCGCAC59200.010.3884235
CGCACAT59850.010.20057637
ACTATTA57000.09.70373619
ACACGTC70450.09.57475413
ATCGGTG87800.09.5257796
Produced by FastQC (version 0.11.5)