FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27168898
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45050.024.75672515
CGTCTGA46900.023.6367416
GTCCGCA50950.020.6915436
CTCGTAT41300.019.31205444
CACGTCT59750.018.92892614
TCGGAAG60600.018.2934573
ATCGGAA61000.018.1366372
TCGTATG41450.017.9375845
CACACGT64500.017.53480712
CGCATCT62100.016.50686539
ACCCGTC68900.015.88705332
TCCGCAT66250.015.81145437
ACACGTC77100.015.01915713
TCACCCG73250.014.93535730
CCGTCCG77150.014.48155734
CAGTCAC82250.014.10582527
AGTCACC86500.013.31050528
GTCACCC84500.012.93917329
CGTCCGC88100.012.81008935
GATCGGA90150.012.3728871
Produced by FastQC (version 0.11.5)