FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002194
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48800.026.72404115
CGTCTGA52900.024.52569616
ATCGGAA63100.020.5256982
TCGGAAG65650.020.0024383
CACACGT65800.019.92142112
CACGTCT67750.019.44772114
CTCGTAT34600.018.69038244
ACACGTC71250.018.55539113
TGTCAGA73900.017.34597235
GTCAGAA73300.017.3035536
GTCACAT77800.016.51250329
AGTCACA78700.016.25764828
CAGTCAC84000.015.97925327
TCGTATG34450.015.82838945
GATCGGA86650.015.0782311
CGGAAGA91000.014.6527164
CCAGTCA91800.014.2779426
CACATGT92850.014.11596431
TCCAGTC96250.013.78132425
AATCTCG69900.013.76435541
Produced by FastQC (version 0.11.5)