FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26785156
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG36800.026.26287734
TCCGTAT38050.025.57954437
CGTATCT37050.025.11695339
CCGTATC39800.024.22910738
ACGTCTG42900.023.37522515
CGTCTGA45300.022.13709316
CTCGTAT36250.022.0051744
TCGTATG37300.020.53988345
TTCCGTA47600.020.3522836
GTTCCGT53850.018.15744435
CACGTCT59000.017.4152414
TCGGAAG59250.017.228853
ATCGGAA59900.016.7791332
TATCTCG53950.016.58152841
CACACGT62600.016.52128812
GTCACAG67000.015.0101829
TCACAGT69200.014.51033230
ACAGTTC70950.014.44609332
AGTCACA70400.014.11744928
CACAGTT72700.014.064054531
Produced by FastQC (version 0.11.5)