FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l03_n01_A3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l03_n01_A3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24700518
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35700.024.5632515
CGTCTGA40200.021.81402816
CTCGTAT34150.021.18026544
TCGTATG35300.019.91572445
CACGTCT53100.016.97951114
CACAGTC52050.016.78660831
TCGGAAG54500.016.5030213
ATCGGAA54300.016.3153822
CACACGT54750.016.26226812
GTCACAG57900.014.9599829
AGTCAAC60250.014.76865234
TCACAGT62400.014.2144430
AGTCACA65950.013.46803528
ACACGTC69300.013.431890513
AATCTCG63050.013.18713541
CAGTCAC71350.012.98286827
ACAGTCA78100.011.96881632
GTCAACA74550.011.93612935
AGCACAC79900.011.902884510
GATCGGA77500.011.6063141
Produced by FastQC (version 0.11.5)