FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523136
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT7100.023.4354972
TCTCGGT3500.021.9295437
AGTGTAG3400.021.13529429
GAGTGTA3600.019.96119128
TAGATCT4100.019.7170533
GTGTAGA3800.019.50077830
GTAGATC4150.018.93832432
GATCTCG5350.016.3702435
GTGTAGG5400.015.80653217
ATCGGAA9300.014.99027252
TTCCCTA4800.014.9677768
TCGGTGG5600.014.15475739
TCGGAAG9850.014.1533663
TCGTGTA5750.014.05860815
TCCCTAC4200.013.8979849
TGTAGAT5350.013.85063231
AGCGGTT3750.013.76985910
CTCGGTG6300.013.65291338
AGATCTC6150.013.51031934
GATCGGA10350.013.4689811
Produced by FastQC (version 0.11.5)