FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32837746
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT30250.015.4137662
GCGTGAC45450.011.249621
GTAGAAC44950.010.85546211
CTCTATA68400.09.77779245
GTGACTC51300.09.6161333
CACTCGT69650.09.55945735
ACTCGTG70800.09.4058936
TCACTCG69400.09.39925134
GTACTCG62550.09.3980524
GGAAAAT140900.09.1758591
GAATCGA48350.08.8042014
TAGAACA55850.08.77721812
GCGGAAA87500.08.752191
TACTCGA66100.08.687815
AAAATCC105900.08.6358253
CTAGCAC58650.08.51280614
AATCGAA56300.08.48006255
TCAGGTA60150.08.3375897
GGTAGAA61850.08.10726110
GCTCTAG62300.07.97668611
Produced by FastQC (version 0.11.5)