FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32433474
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC56000.010.68108311
GGAAAAT166000.08.8333051
AAAATCC121300.08.8021083
TCAGGTA73350.08.7074757
TAGAACA71400.08.56636112
CAGGTAG70700.08.5238748
GGTAGAA73700.08.32929310
AATCGAA62250.08.2484935
GAATCGA53550.08.159634
AAATCCT127200.08.0389054
AATTGAC53400.07.97075945
GGGGAAT70050.07.84570551
CGTTAAC32900.07.72754621
CGAAATT48750.07.5315692
TCCGTTA36850.07.448612719
GTGTGAG84150.07.430159615
GGTAAGT31600.07.40467517
AATCCTT108650.07.399765
GGGAAAT84400.07.39243751
GAATTGA85100.07.35772234
Produced by FastQC (version 0.11.5)