FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37659985
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC53650.010.68769611
GTACTCG58150.08.8694754
GGAAAAT152000.08.8020291
TCAGGTA70850.08.7288897
CACTCGT52500.08.65491435
TACTCGA62450.08.5463945
GGTAGAA71150.08.18521110
GAATCGA64200.07.9634824
CAGGTAG74250.07.90420638
TAGAACA76400.07.88784812
AAAATCC106150.07.8514413
TCACTCG58250.07.76169134
GGGAAAT96400.07.71039871
GCGGAAA72150.07.4922941
CTCGAAT37950.07.4782271
AAAAATC114600.07.4099882
CGGAAAT33600.07.3738331
AATCGAA67400.07.31730655
CGTGACT26500.07.3099322
AGAACGG256500.07.2624953
Produced by FastQC (version 0.11.5)