FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29215448
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44400.010.38231311
CGTGACT20550.09.9743242
CACTCGT45050.09.03742535
GGTAGAA52950.08.9604410
TAGAACA53350.08.89359212
GGAAAAT116550.08.8702471
TCAGGTA57050.08.8695947
GTACTCG42650.08.60767654
CAGGTAG57750.08.5663728
AAAATCC85550.08.5308953
GAATCGA45550.08.4552244
AATCGAA57750.08.4231485
ACTCGTG47900.08.31342236
TCACTCG49400.08.195888534
AGAACGG200100.08.0825233
TACTCGA45200.07.97176075
GGGAAAT66700.07.93555551
AAAAATC82350.07.9047512
CGTAGGT15750.07.854943835
CTCTATA53050.07.768068345
Produced by FastQC (version 0.11.5)