FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26518219
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23350.012.2508492
GTAGAAC41200.011.24349511
GTACTCG47950.09.7698514
TCAGGTA51050.09.5596157
GCGTGAC33550.09.53307151
GAATCGA42300.08.9450374
TACTCGA51800.08.8253155
TAGAACA52050.08.77027512
GGAAAAT113500.08.7514451
GGTAGAA53450.08.66641210
CACTCGT43450.08.43835735
AATCGAA53100.08.3972025
AAAATCC83900.08.2689813
AGTACTC63050.08.2525883
CAGGTAG57250.08.2091478
GATCTAG44700.08.0103885
ATCTAGC46350.07.81411366
CTCGAAT23550.07.74695731
GTGACTC39850.07.74382543
CTCTATA46750.07.70708245
Produced by FastQC (version 0.11.5)