FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31582793
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32450.017.0756232
GCGTGAC46600.012.1802741
GTGACTC51450.011.3830683
GTAGAAC45700.011.36613211
GTACTCG64650.010.6604954
TACTCGA67000.010.3526145
CTAGCAC57200.09.947288514
TCAGGTA56900.09.8414027
GCTCTAG61950.09.58251511
AGTACTC80750.09.2054023
AATCGAA62650.09.094395
TGACTCT68750.09.0091634
TAGAACA57350.08.90050912
CACTCGT58200.08.85061235
GGTAGAA60450.08.74139810
GGAAAAT135850.08.7210451
CTCTATA59700.08.71315145
GAATCGA51000.08.61174
ACTCTGC79050.08.5087266
AAAATCC104550.08.3163863
Produced by FastQC (version 0.11.5)