FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27741999
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25350.015.3720452
GCGTGAC35400.012.2165151
GTAGAAC39250.011.34321211
GTACTCG50800.010.0641834
GTGACTC43850.09.8116013
GAATCGA43250.09.7901234
CTCTATA56350.09.55105545
CACTCGT56750.09.23546835
TACTCGA54900.09.1474715
TAGAACA50650.09.01230112
ACTCGTG59600.08.9840436
GGTAGAA51200.08.915210
TCAGGTA53750.08.744487
AATCGAA51600.08.7286855
TCACTCG61600.08.58110934
AGTACTC65200.08.3952713
TGACTCT55950.08.37297254
GCTCTAG51400.08.355659511
GGAAAAT117850.08.161081
AAAATCC88300.08.1377823
Produced by FastQC (version 0.11.5)