FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37105022
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT34300.014.7760662
GCGTGAC47450.011.20271
GTAGAAC49700.010.94903311
CTCTATA70200.09.3347745
GGAAAAT165150.09.151471
GTGACTC57450.09.0571913
CACTCGT72050.08.92879935
CTCGAAT35100.08.9197891
GTACTCG69200.08.852894
TCAGGTA68500.08.7657747
TACTCGA69800.08.6467395
AAAATCC116300.08.6382693
TAGAACA63900.08.62162312
TCACTCG76050.08.54766134
GAATCGA54950.08.5254624
ACTCGTG76650.08.36513136
GGTAGAA65450.08.31405910
AATCGAA66850.08.2198025
GCGGAAA87800.08.1835751
CTAGCAC66750.08.11996414
Produced by FastQC (version 0.11.5)