FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l02_n02_N3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n02_N3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31317837
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31650.016.3690152
GCGTGAC46250.011.9805431
GTGACTC52600.010.5348983
CTCTATA64300.09.91115645
GTAGAAC39450.09.74669511
CACTCGT65350.09.63782535
CTAGCAC56950.09.63536614
GAATCGA46650.09.5595664
ACTCGTG68650.09.30717836
GCTCTAG59200.09.30577711
TCACTCG69100.09.11452534
AATCGAA58300.09.0002875
GTACTCG58450.08.9012734
GCGGAAA84050.08.7632091
TACTCGA58450.08.7460175
TGACTCT70500.08.4980094
GGAAAAT128500.08.2560231
AAAATCC98550.08.114333
TCAGGTA52550.08.0453477
ACTCTGC78500.07.8519476
Produced by FastQC (version 0.11.5)