FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523136
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4600.035.19306615
CGTCTGA4500.034.9763816
CACACGT5600.028.9074312
CACGTCT5750.028.54468514
ACACGTC6250.026.62085213
CCAGTCA5700.023.27793726
CAGTCAC5150.022.70719527
AGCACAC8550.019.72257810
AGACCGA4250.019.59778632
TCGGAAG11700.018.6637633
ATCGGAA11500.018.5906682
GAGACCG4150.018.44382931
GCACACG9450.017.3682511
CCGAGAC4650.016.93250529
ACCGATC4400.016.88626734
CGGAAGA13350.016.694334
GATCGGA13150.016.4297921
CTCCAGT8900.015.91640924
AACTCCA10150.015.06311722
AGAGCAC12750.013.0537118
Produced by FastQC (version 0.11.5)