FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32837746
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG356380.10852754631819127TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC77050.031.65633838
ACGTCTG83100.031.58226815
CGTCTGA83750.031.31096616
CCGTACG84150.030.53337133
ACCGTAC91700.028.28620732
TCGTATG64100.028.00640945
CTCGTAT70750.027.31609744
CACCGTA95950.026.72375931
CACGTCT104750.025.39722814
ATCGGAA102800.025.290422
TCGGAAG104250.025.0896343
CACACGT107200.024.62731212
TACGTAA102150.024.18421636
GTAATCT100950.024.16240739
ACGTAAT102700.023.92408237
CAGTCAC114050.022.79325127
TAATCTC115700.021.0247940
AATCTCG112500.020.36202641
ACACGTC133650.020.359713
AGTCACC131100.020.17267828
Produced by FastQC (version 0.11.5)