FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32433474
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG50350.034.21433333
AGTCACG59200.029.89571228
ACGTCTG61200.029.6910715
GTTTCGG64300.026.75760734
CGTCTGA68950.026.25642216
ACGTTTC68300.025.54925232
TTCGGAA65950.025.405536
TTTCGGA67950.024.95577635
GTCACGT76500.023.20128429
ATCGGAA78250.023.0508752
CACACGT81500.022.40482712
TCGGAAT74000.022.27732537
TCGGAAG82500.021.8906333
CACGTCT90600.020.40306914
CTCGTAT45300.019.24306344
CGGAATC86200.019.07348838
CACGTTT93000.018.88062331
ACACGTC100550.018.80897313
CAGTCAC102800.018.04695727
TCGTATG45750.017.4290245
Produced by FastQC (version 0.11.5)