FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37659985
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG72100.029.25618228
ACGTCTG76350.028.01151515
CGTCTGA82000.026.21885716
CACGTGG82600.025.45565231
GTCACGT86950.024.13821629
GGCCTTA89350.023.18835636
CTCGTAT65050.022.64705344
TCGTATG62500.022.05725745
GCCTTAT92650.021.99865237
ATCGGAA97250.021.6689342
CACGTCT100050.021.66735814
TCGGAAG98000.021.6177583
TCACGTG97400.021.55774530
ACGTGGC100150.021.1113132
CACACGT102800.021.10916312
TATCTCG85050.020.49941841
CAGTCAC117150.018.46583727
CTTATCT109950.018.39598739
ACACGTC123500.018.19049613
CCTTATC115250.017.97953838
Produced by FastQC (version 0.11.5)