FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29215448
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG33850.021.25892428
ACGTCTG38050.019.74033215
CGTCTGA40650.018.7547916
TCGTATG37150.018.25040845
CTCGTAT37050.017.63093644
CGTGAAA41750.017.26014733
ACGTGAA45500.016.13292932
CACGTGA47000.015.66295331
AACGATC43850.015.61452138
GTCACGT48000.015.51268329
TGAAACG49850.014.63693635
CACGTCT56200.013.68472514
CACACGT59050.013.40479312
TCGGAAG57100.013.3523193
TCACGTG57750.013.210896530
ATCGGAA56950.013.150542
AAACGAT57700.012.29505337
GAAACGA62550.011.62924536
CAGTCAC70050.011.01089427
TATAGGC65600.010.79799927
Produced by FastQC (version 0.11.5)