FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26518219
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG31150.021.5144415
AGTCACG30550.021.4212428
CGTCTGA34700.019.11910816
ACGTCCG36300.017.98761732
GTCCGCA37000.017.5891534
CTCGTAT33700.017.11083244
TCGTATG33550.016.18023345
GTCACGT44450.014.92985529
CCGCACA43850.014.84158436
ATCGGAA47900.013.991732
TCGGAAG49400.013.9310913
CACACGT51100.013.86248312
CACGTCT52250.013.256314
TCACGTC52250.012.8352930
CAGTCAC58250.012.00648227
TCCGCAC55550.011.95877435
ACACGTC67750.011.15288113
CGCACAT62000.010.4246637
GTCGGTT22050.010.3020761
ATCGGTG93150.09.6085016
Produced by FastQC (version 0.11.5)