FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31582793
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44850.023.81764215
CGTCTGA46750.022.8017316
GTCCGCA53800.019.12959136
ATCGGAA59650.017.2681052
CTCGTAT41800.016.59736444
CACGTCT66400.016.56125614
TCGGAAG64200.016.359533
CACACGT69600.015.73494912
TCGTATG41450.015.70497945
ACCCGTC69250.015.4444832
CGCATCT66450.014.43914439
CAGTCAC75950.014.27132227
TCCGCAT75150.013.72541437
TCACCCG79750.013.31934730
ACACGTC92750.012.583590513
AGTCACC88200.012.41700928
CCGTCCG92800.012.27904634
CCCGTCC89050.012.11289233
GTCACCC92250.011.53431129
CCAGTCA96100.011.39594826
Produced by FastQC (version 0.11.5)