FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27741999
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG69250.031.6638215
CGTCTGA72200.030.43275616
ATCGGAA84250.025.7608472
TCGGAAG85350.025.5076623
CACGTCT88200.025.24214614
CACACGT90100.024.5594312
GTCAGAA88950.023.7977636
CAGTCAC94000.023.20554727
TGTCAGA93100.023.07534635
AGTCACA94950.022.80844528
GTCACAT98700.021.90280729
CTCGTAT33650.021.55383544
ACACGTC114750.019.8133613
CCAGTCA110650.019.7543526
ACATGTC116200.019.26009632
ATGTCAG115250.019.08987634
TCACATG116750.018.87150630
AGCACAC119450.018.80733510
CACATGT117400.018.79134231
GATCGGA118600.018.5467831
Produced by FastQC (version 0.11.5)