FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37105022
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG47750.026.64721934
TCCGTAT48150.025.86588537
CGTATCT48050.025.21933739
CCGTATC50250.024.74217438
ACGTCTG54100.024.23431815
CGTCTGA57750.023.01449416
CTCGTAT45850.020.58509644
TTCCGTA60750.020.53732136
TCGTATG45900.019.18855745
GTTCCGT68700.018.45566235
ATCGGAA76150.017.1294462
TCGGAAG78200.016.939193
CACGTCT81100.016.7478114
CACACGT82550.016.26270512
TATCTCG74000.015.00684341
CAGTCAC91450.014.50787527
AGTCACA93750.014.36832928
CACAGTT94700.014.12077631
TCACAGT92450.014.11891730
GTCACAG94350.014.11504229
Produced by FastQC (version 0.11.5)