FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l02_n01_N3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l02_n01_N3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31317837
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG338480.1080789838710764TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG81350.030.79645315
CGTCTGA82350.030.2591616
CTCGTAT64150.028.65136144
TCGTATG64650.027.3845645
CACAGTC97400.025.12393631
ATCGGAA99400.024.8661562
TCGGAAG101300.024.5995393
CACGTCT102900.024.45496214
CACACGT104550.024.0686712
GTCACAG107700.022.74637429
AGTCAAC107600.022.72871434
CAGTCAC109550.022.47748827
TCACAGT110550.022.23024630
AGTCACA110650.022.11247628
AATCTCG103100.020.90750941
ACAGTCA122150.020.66358232
ACACGTC125450.020.39960513
CCAGTCA124450.019.85860826
GTCAACA124700.019.82868435
GATCGGA133700.018.6053141
Produced by FastQC (version 0.11.5)