FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13001660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN413130.31775173324021705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5800.025.9523582
AGTGTAG2850.017.33531229
GAGTGTA2900.017.03754628
GTAGATC3300.017.01092332
TAGATCT3550.015.81352233
TAGCGTA1056.157908E-414.96273810
TCTCGGT3800.014.84781737
GTGTAGG4550.014.31874317
TCGTGTA4250.013.73602615
TCGGAAG8200.013.69706253
AGTTAGG1000.007628075313.4807741
TAGGGAA4700.013.40225620
GTAGGGA4700.013.402119
GTGTAGA3800.013.00022930
ATCGGAA8800.012.7648982
TCCGATT3355.456968E-1212.743021
TTCCCTA3700.012.739588
GATCTCG5800.012.390655535
AATAGCG1300.00307937612.0862678
CGGAAGA9900.011.7988874
Produced by FastQC (version 0.11.5)