FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28588422
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28500.015.239562
GCGTGAC40350.011.6007441
GTAGAAC35500.011.02008611
GTGACTC47000.010.39022353
TACTCGA54950.09.8693375
GTACTCG53500.09.8008824
AAAATCC83550.09.2925663
GGAAAAT115150.09.0095491
CTAGCAC52000.08.99568814
AATCGAA53900.08.9761135
CTCGAAT25000.08.9117141
CACTCGT62850.08.84069535
GAATCGA43550.08.6812874
TGACTCT59850.08.5730174
GAATTGA67550.08.5285984
AGTACTC65800.08.4818153
GGTAGAA48800.08.47741810
GCTCTAG57950.08.4191911
TCACACG34750.08.41507815
TCAGGTA51000.08.3344467
Produced by FastQC (version 0.11.5)