FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30478389
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA49950.012.9303044
CTAATCG45600.012.3378743
GTAGAAC51400.011.32910211
AATCGAG75500.010.431785
GGTAGAA62950.09.17895810
GAATCGA48550.09.0850884
TAGAACA64700.08.89640412
GCTAATC68600.08.857242
AAAATCC102500.08.7821593
GGAAAAT141200.08.7339571
CAGGTAG67900.08.4773568
GGTAAGT25550.08.45386817
TCAGGTA73150.08.2085627
CGAATCG33100.08.1586523
AGGTAGA74800.07.99533569
AAAAATC92450.07.98409462
AATTCAC49500.07.8191695
ATTAGGG26800.07.8072416
AGTTCCG55500.07.58226221
GCGAAAT65750.07.56417751
Produced by FastQC (version 0.11.5)