FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36104271
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG70200.011.79686454
GTAGAAC54750.011.33440411
TACTCGA74350.010.7444455
CGTGACT27050.010.5654682
AGTACTC87650.09.5509713
GGTAGAA68700.09.06562910
TCAGGTA74300.08.9294197
AATCGAA71150.08.8872625
GAATCGA66200.08.3964344
TAGAACA75950.08.23014612
GGAAAAT137100.08.1415091
CAGGTAG79850.08.1101568
CTCGAAT37300.08.0845651
AGAACGG239200.07.76156433
GCGTGAC43300.07.63994361
AAAAATC110200.07.51481872
AACGGCC165750.07.48056945
AGATCAG135800.07.3079364
AAAATCC101050.07.30455883
GGGAAAT91100.07.23785641
Produced by FastQC (version 0.11.5)