FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29108556
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26850.012.6559092
GTAGAAC42900.011.32057211
GTACTCG54200.010.0896454
TCAGGTA52850.09.8725147
TACTCGA58150.09.6747015
GGAAAAT118400.09.2562951
GGTAGAA52600.09.23296910
GCGTGAC39400.09.1958021
AATCGAA54050.09.1595525
GAATCGA50400.08.9303474
AAAATCC90800.08.7489113
TAGAACA56000.08.63260312
AGTACTC67650.08.5825643
AATTGAC45550.08.3986175
GAATTGA71150.08.3502214
CAGGTAG62100.08.1469488
TCACACG36550.08.1237515
GTGACTC46150.07.89966153
AGAACGG191650.07.7617183
GAAAATC98950.07.73256872
Produced by FastQC (version 0.11.5)